The following is a list of all characters appearing in Arby 'n' the Chief.
- Master Chief (Microsoft Sam) is the foul-mouthed, illiterate protagonist of the series next to the Arbiter.
- Arbiter (Microsoft Mike) is the friendly and intelligent protagonist of the series next to Chief.
- Cortana (Microsoft Mary) was the original tritagonist of the series and was also briefly the tritagonist of Season 7 alongside Greg.
- Todd (Jon Graham)
- Travis (Jon Graham)
- Claire Ferguson (Elizabeth Carr-Ernst) is a fomer tritagonist of the series and Arbiter's love interest.
- Greg is the mute and friendly spider that Arbiter befriends in "The Spider" and the tritagonist of Season 5 and Season 7.
- Skyler Loveheart (Jon Graham) is the head of Douchebag Studios. Skyler invites Master Chief to Los Angeles to work for his company to produce Machinima.
- Craig (Jon Graham)
- Unnamed Cheater (Jon Graham) is a hacker that Chief encounters online.
- Zanthar (Jon Graham)
- Trent Donnovich (Jon Graham) is the main antagonist of Seasons 5 and 6.
- Victoria Donnovich (JC Cheng)
- Brittany Donnovich (Elizabeth Carr-Ernst)
- Cameron Jones (Ian Beckman)
- Cody Hammond (hollywoodangels)
- Brian (Ron Campbell)
- Chaos Theosis is an infamous group of hackers that aims to disband TOSERS.
- Clyde (Jon Graham) is the primary antagonist of Season 6 and the leader of Chaos Theosis. He is secretly aiming to take down the Online Multiplayer Network for money.
- Kylie (Elizabeth Carr-Ernst) is the secondary antagonist behind Clyde in the sixth season. She is helping Clyde so that she will be able to start a new life away from her abusive father.
- Kylie's bodyguards (Ian Beckman and Matt Dannevik) are a pair of players that admire Kylie and help to protect her in Severance.
- Adam McIntyre (Khail Anonymous) is the tertiary antagonist of the sixth season. He is a malicous eight year old that joined the group for his enjoyment of making people suffer.
- Duncan (Hutch) is the fourth antagonist of the sixth season. He is an MLG player that joined to preserve online freedom by disbanding TOSERS.
- Other Hackers:
- Hacker Leader (Season 6) (Unknown) is the leader of a group of four hackers that is distributing Fragban to players on private networks.
- Hacker Grunt (Kid) (Jon Graham) is a younger member that spends most of his time arguing with other kids on the privete networks.
- Hacker Grunt (Red) (Unknown) is a member of the group with red armour and guards the server that the hackers use.
- Hacker Grunt (Grey) (Unknown) is a member of the group that checks gamertags of players that attempt to make an exchange to see if they are affiliated with TOSERS.
- Justin (Unknown) is a software engineer that developed the hacks used by the Underground Hackers and by Chaos Theosis and is making a deal with Trent Donnovich.
- Eugene Black's Clan
- Jon (Jon Graham)
- See article: Jon (character)
- Xbox Live Players (Various)
- See article: Xbox Live Players
- Randall (Jon Graham)
- Randall is a plush that apparently filmed the Bytes episode "Doggy Style". He is thanked by Arbiter for his terrific job at filming them. Randall parodies Master Chief's idea of introducing a new character at the end of his version of Mass Effect 3's ending, and possibly parodies the Catalyst in Mass Effect 3 itself.
- Gregory (Jon Graham)
- Sarah (Jon Graham)
- Krystal (Jon Graham)
- Charlie (Jon Graham)
- Red Player (Jon Graham)
- Blue Player (Jon Graham)
- Josh Butterballs (Jon Graham)
- mastur cheef (Daniel Laszlo)
- teh arbitur (Daniel Laszlo)
- Butt Beard (Jon Graham)
- FEELS GOOD MAN (Jon Graham)
- Boris Smirnoff (Daniel Laszlo)
- Timothy (Jon Graham)
- Nathan (Daniel Laszlo) is a member of Bad Programming, the media broadcasting company associated with Trent Donnovich.
- Daniel (Jon Graham)
- Unnamed Construction Team Member
- Underground Hackers
- Jon GJC (SeaNanners)
- Allen Radcliffe (Jon Graham)
- Mrs. McIntyre (Lindsey Washburn)
- Gordon Jones (Jon Graham)
- blumpkinbeast666 (Jon Graham)
- Emma (Jennifer Espino)
- Madeline Black
- Michelle Harris (Jennifer Espino/Elizabeth Carr-Ernst)
- Brody Mitchell (Jon Graham)
- Kyle Mathison (Matt Dannevik/Kris Boruff)
- Blake (Khail Anonymous)
- Dr. Harris (Jon Graham)